Researchers from the AI-AGE team participate in the he 34th EUROPEAN ASSOCIATION FOR DIABETIC EYE COMPLICATIONS (EAsDEC) Meeting, which was hosted in the city of Milan, Italy. Initial research results from the AI-AGE project were presented in a form of a poster by prof. N Popovic and prof. A. Adzic Zecevic. The conference took place from 30 May to 1 June. More information can be found here.
ABSTRACT – Introduction / study design: Type 2 diabetes mellitus (T2D) is associated with changes in retinal microvascular complexity measured by fractal dimension (Df). Expression of micro RNAs (miRs), -146a and -101 is also affected by T2D, but the studies investigating these miRs in context of microvascular changes in T2D are scarce. Since hypertension (HTN) and Alzheimer’s dementia (AD) frequently coexist in patients with T2D, in the present cross-sectional observational prospective study, participants were divided in two groups – healthy (H, n=8), and with chronic disease (D, n=20, suffering from T2D, HTN and/or AD). The purpose: Study explores association between changes of retinal microvascular Df and expression levels of circulating miR-146a and miR-101 in patients with T2D. The influence of HTN and AD on this association is also investigated. Methods: Retinal fundus images were captured by using a non-mydriatic, hand-held MIIS-HORUS scope DES200. The optic disc-centered images were manually segmented, binarized, cropped to 350-pixel radius, and Df was determined by using ImageJ 1.53q. MiRs were isolated from plasma, quantified by qRT-PCR and normalized to expression levels of miR-361-5p. SPSS Statistics 29.0.1.0., t-test and ANCOVA were used to compare the two groups. P<0.05 was considered significant. Results: Age was not different between the 2 groups (Hvs.D mean age±SE=63.6±2.9 vs.68.5±1.8, p=0.17). Df and miR-101 expression were decreased in the group D (Hvs.D mean: Df±SE=1.36±0.01 vs.1.32±0.01, p=0.016; miR-101±SE=1.68±0.32 vs.0.83±0.22, p=0.041). Eight participants in the group D had T2D (1-moderate, and 7-no diabetic retinopathy). All participants with T2D had HTN, and 5 of them also had AD. Next, we used HTN and AD as covariates to account for effects of these comorbidities, and to determine effects of T2D. This analysis showed: in addition to decreased Df and miR-101 expression, T2D was associated with increased expression of miR-146a (Hvs.D mean miR-146a±SE=0.58±0.29 vs.1.69±0.17, p=0.018). Conclusions: fa Changes in Df and in expression of miR-101 are non-specific, and can be caused by T2D and concurrent comorbidities. Increased expression of miR-146a might be a part of the unique expression pattern of the circulatory miRNAs associated with T2D.
